Postdoctoral opportunity at the Butantan Institute's Laboratory of Gene Expression in Eukaryotes to study the molecular mechanisms of action of the long non-coding RNA PVT1 (lncRNA PVT1) in a prostate cancer cell line, under the supervision of Professor Sergio Verjovski-Almeida. The selected candidate will live and work in São Paulo city, Brazil.
The aim is to understand the large-scale regulation of prostate cancer gene expression activation and inhibition programs, exploring the recent observation of our group that PVT1 is associated with the androgen receptor (AR) in prostate cells.
Advanced molecular biology tools will be used, such as lincRNA PVT1 silencing with gapmers followed by ChIP-Seq, to map in the genome the genes that are the target of epigenetic modulation by AR and PVT1, as well as ChIRP-MS and fluorescence microscopy to reveal possible new partners of the PVT1-AR complex in the prostate.
The candidate must have obtained a PhD in Biological Sciences with emphasis on Biochemistry and Molecular Biology less than five years ago, publications in the area, good communication skills, experience in cell line cultivation, RT-qPCR, western blottings and immunohistochemistry.
This opportunity is open to candidates of any nationalities. The selected candidate will receive a Post-Doctoral fellowship from the São Paulo Research Foundation (FAPESP) in the amount of R$ 7,373.10 monthly and a research contingency fund, equivalent to 15% of the annual value of the fellowship which should be spent in items directly related to the research activity.
More information about the fellowship is at: www.fapesp.br/oportunidades/3343/en